/
/
Indução de Murino inflamação intestinal por transferência adotiva de Effector CD4<sup> +</sup> CD45RB<sup> Alta</sup> Células T em imunodeficientes Mice
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
1Center for Gastrointestinal Biology and Disease,University of North Carolina at Chapel Hill, 2Department of Microbiology and Immunology,University of North Carolina at Chapel Hill, 3Department of Genetics,University of North Carolina at Chapel Hill, 4Curriculum in Genetics and Molecular Biology,University of North Carolina at Chapel Hill
Capítulos
- 00:05Título
- 01:18Isolation of Splenic T Cells from Donor Mice
- 02:41Enrichment of CD4+ T Cells
- 04:34Labeling and Sorting the CD4+ T Cells
- 06:31Injection of Cells into Recipient Mice and Monitoring of Disease Progression
- 07:22Results: Colitis Progression After Adoptive T Cell Transfer into Recipient Mice
- 08:15Conclusion
Here, we present a protocol to induce colonic inflammation in mice by adoptive transfer of syngeneic CD4+CD45RBhigh T cells into T and B cell deficient recipients. Clinical and histopathological features mimic human inflammatory bowel diseases. This method allows the study of the initiation of colonic inflammation and progression of disease.
