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Biologia do Desenvolvimento
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Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone

DOI:
10.3791/52544-v

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09:31 min

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April 08, 2015

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, , , ,
1Immunodynamics,German Rheumatism Research Center, a Leibniz Institute, 2Biophysical Analytics,German Rheumatism Research Center, a Leibniz Institute, 3Max-Delbrück Center for Molecular Medicine, 4Wimasis GmbH, 5Immunodynamics and Intravital Imaging,Charité – University of Medicine

Capítulos

  • 00:05Título
  • 01:14Femoral Bone Cryosectioning
  • 02:28Imaging
  • 04:02Random Bone Marrow Positioning Simulation
  • 07:36Results: Representative Analysis of the Bone Marrow Niche Microenvironments for Long Lived Plasma Cells
  • 09:00Conclusion

Summary

Tadução automática

Tadução automática

A strategy to quantitatively analyze histological data in the bone marrow is presented. Confocal microscopy of fluorescently labeled cells in tissue sections results in 2-dimensional images, which are automatically analyzed. Co-localization analyses of different cell types are compared to data from simulated images, giving quantitative information about cellular interactions.

Tags

Automated QuantificationCell-cell InteractionsHematopoietic CellsStromal CellsBone MarrowConfocal MicroscopyHistological AnalysisCell RecognitionNeighborhood AnalysisCo-localizationRandom Positioning

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