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Isotérmico de amplificación (LAMP) Ensayos Loop mediada para la detección específica de las especies de<em> Eimeria</em> Que infectan pollos
JoVE Journal
Imunologia e Infecção
This content is Free Access.
JoVE Journal Imunologia e Infecção
Loop-mediated Isothermal Amplification (LAMP) Assays for the Species-specific Detection of Eimeria that Infect Chickens
DOI:

06:57 min

February 20, 2015

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Capítulos

  • 00:05Título
  • 01:16Template Preparation
  • 02:44Eimeria LAMP Primer Preparation
  • 03:50Eimeria LAMP Assay and Read-out
  • 05:22Results: Eimeria LAMP Assay Testing
  • 06:30Conclusion

Summary

Tadução automática

Diagnosis of Eimeria infection in chickens remains demanding. Parasite morphology- and host pathology-led approaches are commonly inconclusive, while molecular approaches based on PCR have proven demanding in cost and expertise. The aim of this protocol is to establish loop-mediated isothermal amplification (LAMP) as a straightforward molecular diagnostic for eimerian infection.

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