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JoVE Journal Bioengineering
Generation and Grafting of Tissue-engineered Vessels in a Mouse Model
 

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model

Article DOI: 10.3791/52565-v • 13:04 min • March 18th, 2015
March 18th, 2015 •
*1, *1, 1, 1, 1
1Cardiovascular Division, King's College London BHF Centre
* These authors contributed equally

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Resumo
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Here, we present a protocol to generate tissue engineered vessel grafts that are functional for grafting into mice by double seeding partially induced pluripotent stem cell (PiPSC) - derived smooth muscle cells and PiPSC - derived endothelial cells on a decellularized vessel scaffold bioreactor.

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Keywords: Tissue Engineering Vascular Graft Vascular Conduit Decellularized Scaffold Bioreactor Induced Pluripotent Stem Cells Endothelial Cells Smooth Muscle Cells Regenerative Medicine Cell Therapy
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