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Biologia do Desenvolvimento
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Mosaic Zebrafish Transgenesis for Functional Genomic Analysis of Candidate Cooperative Genes in Tumor Pathogenesis
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Mosaic Zebrafish Transgenesis for Functional Genomic Analysis of Candidate Cooperative Genes in Tumor Pathogenesis

Mosaic Zebrafish Transgenesis for Functional Genomic Analysis of Candidate Cooperative Genes in Tumor Pathogenesis

DOI:
10.3791/52567-v

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09:45 min

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March 31, 2015

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, , , ,
1Department of Molecular Pharmacology & Experimental Therapeutics,Mayo Clinic College of Medicine, Center for Individualized Medicine, 2Tufts University School of Medicine, 3Department of Biochemistry and Molecular Biology,Mayo Clinic

Capítulos

  • 00:05Título
  • 01:21DNA Constructs and Transgenesis
  • 04:10Microinjection
  • 05:24Screen for Stable or Mosaic Transgenic Fish
  • 07:40Results: ALK and MYCN Induced Neuroblastoma Initiation
  • 09:13Conclusion

Summary

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The goal of this study is to demonstrate how the mosaic transgenesis strategy can be used in zebrafish to rapidly and efficiently assess the relative contributions of multiple oncogenes in tumor initiation and progression in vivo.

Tags

Keywords: Mosaic Zebrafish TransgenesisFunctional GenomicsTumor PathogenesisCooperative GenesALKMYCNNeuroblastomaCancer ModelIn Vivo ImagingTransgenic Techniques

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