Journal
/
Neurociência
/
Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina
JoVE Journal
Neurociência
This content is Free Access.
JoVE Journal Neurociência
Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina

Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina

DOI:
10.3791/52588-v

•

09:05 min

•

May 06, 2015

•

, , , , ,
1Institute for Ophthalmic Research,University of Tübingen, 2Graduate School of Cellular & Molecular Neuroscience,University of Tübingen, 3Bernstein Centre for Computational Neuroscience,University of Tübingen, 4Molecular Genetics Laboratory,University of Tübingen, 5Centre for Ophthalmology,University of Tübingen

Capítulos

  • 00:05Título
  • 01:59Eye Enucleation and Isolation of Retina
  • 03:22Slice Preparation
  • 04:59Two-photon Microscopy
  • 06:14Light Stimulation and Recording
  • 06:53Results: Light-evoked Ca2+ Responses in Axon Terminals of Mouse Cone Photoreceptors
  • 08:10Conclusion

Summary

Tadução automática

Tadução automática

We describe a protocol to monitor Ca2+ dynamics in the axon terminals of cone photoreceptors using an ex-vivo slice preparation of the mouse retina. This protocol allows comprehensive studies of cone Ca2+ signaling in an important mammalian model system, the mouse.

Tags

Keywords: Cone PhotoreceptorsCalcium SignalingTwo-photon ImagingRetinal DegenerationTransgenic MouseTN-XL BiosensorVertical Retinal SlicesLight-evoked ResponsesAbsolute Calcium Concentrations

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/52588?article_type=v

Multiple-mouse Neuroanatomical Magnetic Resonance Imaging
check_url/pt/52588?article_type=v

Patch-clamp Capacitance Measurements and Ca2+ Imaging at Single Nerve Terminals in Retinal Slices
check_url/pt/52588?article_type=v

Transretinal ERG Recordings from Mouse Retina: Rod and Cone Photoresponses
check_url/pt/52588?article_type=v

Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo
check_url/pt/52588?article_type=v

Assessment of Vascular Regeneration in the CNS Using the Mouse Retina
check_url/pt/52588?article_type=v

Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging
check_url/pt/52588?article_type=v

Vibratome Sectioning Mouse Retina to Prepare Photoreceptor Cultures
check_url/pt/52588?article_type=v

Whole-mount Imaging of Mouse Embryo Sensory Axon Projections
check_url/pt/52588?article_type=v

In Situ Ca2+ Imaging of the Enteric Nervous System
check_url/pt/52588?article_type=v

Two-photon Imaging of Cellular Dynamics in the Mouse Spinal Cord

Read Article