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Imaging Ca<sup> 2+</sup> Dynamics i Cone Lysmodtageren Axon Terminals i Mouse Retina
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1Institute for Ophthalmic Research,University of Tübingen, 2Graduate School of Cellular & Molecular Neuroscience,University of Tübingen, 3Bernstein Centre for Computational Neuroscience,University of Tübingen, 4Molecular Genetics Laboratory,University of Tübingen, 5Centre for Ophthalmology,University of Tübingen
Capítulos
- 00:05Título
- 01:59Eye Enucleation and Isolation of Retina
- 03:22Slice Preparation
- 04:59Two-photon Microscopy
- 06:14Light Stimulation and Recording
- 06:53Results: Light-evoked Ca2+ Responses in Axon Terminals of Mouse Cone Photoreceptors
- 08:10Conclusion
Vi beskriver en protokol til overvågning Ca 2+ dynamik i Axon terminaler kegle fotoreceptorer bruger en ex-vivo skive forberedelse af musen nethinden. Denne protokol giver mulighed for omfattende undersøgelser af kegle Ca2 + signalering i en vigtig pattedyr modelsystem, musen.
