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Capítulos
- 00:05Título
- 01:23Infection
- 03:38Visualization of Cardiac Lesions and Pneumoccocci within Lesions
- 04:38Immunofluorescent Microscopy for S. pneumoniae within Microlesions
- 06:13Collagen Depostion Detection
- 07:03Results: Cardiac Pathology of S. pneumoniae-challenged and Rescued Mice
- 07:56Conclusion
Streptococcus pneumoniae forms discrete non-purulent microscopic lesions in the heart. Outlined is the protocol for a murine model of cardiac microlesion formation. Instruction is provided on microlesion visualization using microscopy, discrimination between early and late microlesions, and methods to detect cardiac remodeling in hearts of convalescent animals.
