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免疫细胞健康和疾病肝脏使用CXCR6.Gfp记者小鼠长期活体多光子显微镜成像
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice
DOI:

11:44 min

March 24, 2015

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Capítulos

  • 00:05Título
  • 02:50Tracheotomy
  • 04:36Laparatomy
  • 05:50Sample Setup
  • 08:39Results: Intravital TPLSM with CCI4 Damaged Livers
  • 10:02Conclusion

Summary

Tadução automática

Stable intravital high-resolution imaging of immune cells in the liver is challenging. Here we provide a highly sensitive and reliable method to study migration and cell-cell-interactions of immune cells in mouse liver over long periods (about 6 hours) by intravital multiphoton laser scanning microscopy in combination with intensive care monitoring.

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