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单人的生物粒子从法医“触摸DNA'证据回收简体显微增强遗传分析

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

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单人的生物粒子从法医“触摸DNA'证据回收简体显微增强遗传分析

DOI:

10.3791/52612-v

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11:49 min

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March 09, 2015

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Katherine Farash², Erin K. Hanson, Jack Ballantyne^2,3

¹Forensic Science Graduate Program, Biochemistry Track,University of Central Florida, ²Department of Chemistry,University of Central Florida, ³National Center for Forensic Science,University of Central Florida

Capítulos

00:05Título
02:13Collection of Bioparticles from Touched Objects and Surfaces
04:31Optional Staining of Bioparticles to Aid in Visualization
05:33Isolation of Bioparticles of Interest for Analysis
09:05Combined Microvolume Nucleic Acid Isolation and Autosomal STR Profiling
09:51Results: Enhanced Genetic Analysis of Touch DNA Evidence
11:14Conclusion

Summary

Tadução automática

在这里，我们描述了用于生物颗粒存在于收集的优化和有效地去除战略“触摸的DNA的样品，连同涉及一步法5μl的微体积的裂解/ STR扩增增强扩增，以允许恢复短串联重复序列（STR）的生物颗粒体（多个）的轮廓。

Tags

Forensic DNA Analysis Touch DNA Micromanipulation Single-source DNA Profiles STR Amplification Genetic Analysis Bioparticle Recovery Enhanced Techniques Trace Biological Material

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