Journal
/
/
दिमाग़ी Thymic उपकला सेल प्रसार, भेदभाव और अनेक जीन एक्सप्रेशन सहायक 3 डी Organotypic सह संस्कृति मॉडल
JoVE Journal
Biologia do Desenvolvimento
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia do Desenvolvimento
3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression
DOI:

06:47 min

July 30, 2015

, , , ,

Capítulos

  • 00:05Título
  • 01:163D Organotypic Co-cultures (OTCs)
  • 02:46mTEC Co-culture
  • 05:14Results: Growth Patterns and Proliferation of mTECs within the OTCs
  • 06:10Conclusion

Summary

Tadução automática

Studying medullary thymic epithelial cells in vitro has been largely unsuccessful, as current 2D culture systems do not mimic the in vivo scenario. The 3D culture system described herein - a modified skin organotypic culture model - has proven superior in recapitulating mTEC proliferation, differentiation and maintenance of promiscuous gene expression.

Vídeos Relacionados

Read Article