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Capítulos
- 00:05Título
- 01:08PDMS Device Fabrication
- 05:47Cell-free Protein Synthesis: Microfluidic Connections Setup
- 08:25Cell-free Protein Synthesis: Sample Loading and Data Acquisition
- 11:00Image Analysis and Data Processing
- 11:58Results: Stochastic Processes in Gene Networks Can Be Assayed Using Femtoliter Cell-free Protein Synthesis Reactions
- 13:19Conclusion
A microfabricated device with sealable femtoliter-volume reaction chambers is described. This report includes a protocol for sealing cell-free protein synthesis reactants inside these chambers for the purpose of understanding the role of crowding and confinement in gene expression.
