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干血斑点. 免疫和分子技术中使用的制备和加工

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Dried Blood Spots – Preparing and Processing for Use in Immunoassays and in Molecular Techniques

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干血斑点. 免疫和分子技术中使用的制备和加工

DOI:

10.3791/52619-v

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11:28 min

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March 13, 2015

•

Nico Grüner, Oumaima Stambouli, R. Stefan Ross

¹Institute of Virology, National Reference Centre for Hepatitis C, Essen University Hospital,University of Duisburg-Essen

Capítulos

00:05Título
02:43Collection of Blood and Preparation of Blood Spots from Skin Puncture
04:38Drying and Storage of Dried Blood Spots
05:40Elution of Dried Blood Spots and Analysis of Eluates
07:39Results: Testing of Dried Blood Spot Eluates for Markers of Hepatitis B Virus (HBV), Hepatitis C Virus (HCV), and Human Immunodeficiency Virus (HIV) Infections
10:11Conclusion

Summary

Tadução automática

在大多数诊断应用中, 对干血点 (DBS) 进行最终分析的准备和处理仍不规范。为了克服这一缺点, 提出了一个全面的 step-by 步协议, 并随后对其检测病毒感染标志的有效性进行了评估。

Tags

Dried Blood Spots DBS Immunoassays Molecular Techniques Diagnostic Infectious Diseases Newborn Screening Pre-analytical Variables Sample Collection Sample Preparation Sample Drying Sample Storage Sample Transportation Sample Elution Hepatitis B Hepatitis C HIV Automated Analytical Platform

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