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方法培养人股骨组织外植体，究其利基转移性乳腺癌细胞定居

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Medicina

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JoVE Journal Medicina

Methods for Culturing Human Femur Tissue Explants to Study Breast Cancer Cell Colonization of the Metastatic Niche

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方法培养人股骨组织外植体，究其利基转移性乳腺癌细胞定居

DOI:

10.3791/52656-v

•

11:41 min

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March 15, 2015

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Zachary S. Templeton, Michael H. Bachmann, Rajiv V. Alluri, William J. Maloney, Christopher H. Contag, Bonnie L. King

¹Department of Pediatrics,Stanford University School of Medicine, ²Department of Orthopaedic Surgery,Stanford University School of Medicine

Capítulos

00:05Título
02:00Isolating Tissue Fragments from Femoral Heads
02:40Co-culturing to Study Cell Proliferation
04:19Co-culturing to Study Colonization and Cell Number
06:45Measuring Cell Migration in the Co-culture: Towards Bone Tissue Supernatant
09:30Results: Breast Cancer Cell Proliferation, Colonization, and Migration in Co-culture
11:10Conclusion

Summary

Tadução automática

协议描述为研究乳腺癌细胞的迁移，增殖和定植于人体骨组织外植体模型系统。

Tags

Bone Metastasis Breast Cancer In Vitro Model Microenvironment Metastatic Niche Cancer Cell Colonization Bioluminescence Imaging Fluorescence Microscopy Flow Cytometry

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