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Metoder til dyrkning menneskelige femur vævseksplantater at studere Breast Cancer Cell kolonisering af metastatisk Niche
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JoVE Journal Medicina
Methods for Culturing Human Femur Tissue Explants to Study Breast Cancer Cell Colonization of the Metastatic Niche

Metoder til dyrkning menneskelige femur vævseksplantater at studere Breast Cancer Cell kolonisering af metastatisk Niche

DOI:
10.3791/52656-v

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11:41 min

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March 15, 2015

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, , , , ,
1Department of Pediatrics,Stanford University School of Medicine, 2Department of Orthopaedic Surgery,Stanford University School of Medicine

Capítulos

  • 00:05Título
  • 02:00Isolating Tissue Fragments from Femoral Heads
  • 02:40Co-culturing to Study Cell Proliferation
  • 04:19Co-culturing to Study Colonization and Cell Number
  • 06:45Measuring Cell Migration in the Co-culture: Towards Bone Tissue Supernatant
  • 09:30Results: Breast Cancer Cell Proliferation, Colonization, and Migration in Co-culture
  • 11:10Conclusion

Summary

Tadução automática

Tadução automática

Protokoller er beskrevet til at studere brystcancercelle migration, proliferation og kolonisering i et humant knoglevæv eksplantatet modelsystem.

Tags

Bone MetastasisBreast CancerIn Vitro ModelMicroenvironmentMetastatic NicheCancer Cell ColonizationBioluminescence ImagingFluorescence MicroscopyFlow Cytometry

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