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Capítulos
- 00:05Título
- 01:29Preparing for Sample Collection and Processing
- 02:15Tumor Resection and Preparation of Single Cell Tumor Suspension
- 05:47Results: FACS Staining of Single Cell Suspension and Analysis of Leukocyte Subsets
- 07:21Conclusion
This protocol describes a method for the detailed evaluation of leukocyte subsets within the tumor microenvironment in a mouse tumor model. Chemerin-expressing B16 melanoma cells were implanted subcutaneously into syngeneic mice. Cells from the tumor microenvironment were then stained and analyzed by flow cytometry, allowing for detailed leukocyte subset analyses.
