/

/

建立一个人类多发性骨髓瘤异种移植模型中的鸡，研究肿瘤的生长，侵袭和血管生成

JoVE Journal
Medicina

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Medicina

Establishment of a Human Multiple Myeloma Xenograft Model in the Chicken to Study Tumor Growth, Invasion and Angiogenesis

Please note that all translations are automatically generated. Click here for the English version.

建立一个人类多发性骨髓瘤异种移植模型中的鸡，研究肿瘤的生长，侵袭和血管生成

DOI:

10.3791/52665-v

•

10:04 min

•

May 01, 2015

•

Agnieszka Martowicz*^1,4, Johann Kern*^1,2, Eberhard Gunsilius, Gerold Untergasser³

¹Department of Internal Medicine V,Innsbruck Medical University, ²Oncotyrol GmbH, ³Tyrolean Cancer Research Institute, ⁴Division of Vascular Biology, Department of Medical Biochemistry and Biophysics,Karolinska Institute

Capítulos

00:05Título
02:09Cell Culture and Lentiviral Transfection
03:203D-Multiple Myeloma Spheroid Model
04:473D Multiple Myeloma Xenograft Model in the CAM
06:17Quantification of eGFP Protein by ELISA
07:03Immunohistochemical Analysis of Blood Vessels and Invading Tumor Cells
08:05Results: Bortezomib Treatment of Multiple Meyloma Xenografts
09:17Conclusion

Summary

Tadução automática

人多发性骨髓瘤（MM）细胞需要间质细胞和细胞外基质成分为存活和增殖的支持性微环境。我们建立了与植入人类骨髓瘤和间质细胞体内鸡胚模型来研究癌症药物对肿瘤的生长，侵袭和血管生成作用。

Tags

Keywords: Multiple Myeloma Xenograft Model Chicken Chorioallantoic Membrane 3D Culture Angiogenesis Tumor Growth Invasion GFP Reporter Mesenchymal Cells Collagen RPMI-8226 OPM-2 Therapeutic Screening

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

一个<em在体外系统来研究肿瘤休眠和转移性增长

多光子成像在口腔鳞状细胞癌原位小鼠模型的肿瘤细胞的侵袭

一个小鼠模型角膜血管生成研究掌上含量

人类神经内分泌肿瘤细胞株为研究人类神经内分泌肿瘤治疗的三维模型

一种新型的高分辨率<em>在体内</em>成像技术研究肿瘤生长及治疗颅内结构的动态响应

该<em>在OVO</em> CAM作为一个肉瘤Xenograftmodel的检测

视网膜脱离模型鼠害由透明质酸钠的视网膜下注射

一种改进<em>在体外</em>入侵检测，以确定激素，在调节肿瘤细胞侵袭的细胞因子和/或生长因子的潜在作用

注射原位乳腺癌细胞到小鼠乳腺脂肪垫来研究肿瘤的生长。

三维（3D）肿瘤球体侵袭试验

Read Article