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Etablering af en human myelomatose xenograftmodel i kylling til Studieinformationen tumorvækst, invasion og angiogenese
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JoVE Journal Medicina
Establishment of a Human Multiple Myeloma Xenograft Model in the Chicken to Study Tumor Growth, Invasion and Angiogenesis

Etablering af en human myelomatose xenograftmodel i kylling til Studieinformationen tumorvækst, invasion og angiogenese

DOI:
10.3791/52665-v

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10:04 min

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May 01, 2015

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, , ,
1Department of Internal Medicine V,Innsbruck Medical University, 2Oncotyrol GmbH, 3Tyrolean Cancer Research Institute, 4Division of Vascular Biology, Department of Medical Biochemistry and Biophysics,Karolinska Institute

Capítulos

  • 00:05Título
  • 02:09Cell Culture and Lentiviral Transfection
  • 03:203D-Multiple Myeloma Spheroid Model
  • 04:473D Multiple Myeloma Xenograft Model in the CAM
  • 06:17Quantification of eGFP Protein by ELISA
  • 07:03Immunohistochemical Analysis of Blood Vessels and Invading Tumor Cells
  • 08:05Results: Bortezomib Treatment of Multiple Meyloma Xenografts
  • 09:17Conclusion

Summary

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Humane multipelt myelom (MM) celler kræver støttende mikromiljø mesenchymale celler og ekstracellulære matrixkomponenter for overlevelse og proliferation. Vi etablerede en in vivo kylling embryo model med indpodede humane myelom og mesenkymceller at studere virkningerne af kræft lægemidler på tumorvækst, invasion og angiogenese.

Tags

Keywords: Multiple MyelomaXenograft ModelChicken Chorioallantoic Membrane3D CultureAngiogenesisTumor GrowthInvasionGFP ReporterMesenchymal CellsCollagenRPMI-8226OPM-2Therapeutic Screening

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