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Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice
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JoVE Journal Medicina
Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice

Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice

DOI:
10.3791/52675-v

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09:48 min

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May 04, 2015

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, , , , , ,
1Department of Neurology and Neurotherapeutics,University of Texas Southwestern Medical Center, 2Department of Neurological Surgery,Washington University School of Medicine

Capítulos

  • 00:05Título
  • 01:37Repetitive Hypoxic Preconditioning
  • 03:01Evans Blue (EB) Injection
  • 04:222,3,5-Triphenyltetrazolium Chloride (TTC) Staining
  • 08:12Results: RHP Reduces Infarct Volumes of Blood-brain Barrier (BBB) Disruption
  • 08:59Conclusion

Summary

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This protocol describes repetitive hypoxic preconditioning, or brief exposures to systemic hypoxia that reduce infarct volumes and blood-brain barrier disruption following transient middle cerebral artery occlusion in mice. It also details dual quantification of infarct volume and blood-brain barrier disruption after stroke to assess the efficacy of neurovascular protection.

Tags

Keywords: Neurovascular ProtectionRepetitive Hypoxic PreconditioningTransient Middle Cerebral Artery OcclusionInfarct VolumeBlood-brain Barrier DisruptionIschemic StrokeMouse ModelCentral Nervous SystemNeuroprotectionPro-inflammatory Disease

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