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HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues
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JoVE Journal Química
HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues

HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues

DOI:
10.3791/52697-v

•

14:12 min

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August 01, 2015

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, , , , , ,
1Environmental Health Science and Research Bureau,Health Canada

Capítulos

  • 00:05Título
  • 02:28DNA Extraction, Precipitation and Wash
  • 06:06Enzymatic Digestion
  • 07:44Preparation of Standards, HPLC Run, and Quantification
  • 11:19Results: Optimization of 8-oxo-dG Detection and Quantitation
  • 13:10Conclusion

Summary

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The goal of this protocol is the detection of the DNA oxidation marker, 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGuo) by HPLC-ED, in DNA from cultured cells or animal tissues.

Tags

8-oxo-dGuo8-OH-dGuo8-oxo-78-dihydro-2′-deoxyguanosineDNA OxidationOxidative StressHPLC-EDA549 CellsKBrO3Dibenzo(defp)chryseneDBC

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