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Utilization of the Soft Agar Colony Formation Assay to Identify Inhibitors of Tumorigenicity in Breast Cancer Cells
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JoVE Journal Medicina
Utilization of the Soft Agar Colony Formation Assay to Identify Inhibitors of Tumorigenicity in Breast Cancer Cells

Utilization of the Soft Agar Colony Formation Assay to Identify Inhibitors of Tumorigenicity in Breast Cancer Cells

DOI:
10.3791/52727-v

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11:06 min

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May 20, 2015

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, , , ,
1Department of Baker Institute for Animal Health,Cornell University, 2Department of Molecular Biology and Genetics,Cornell University, 3Department of Biochemistry and Molecular Pharmacology,University of Massachusetts Medical School

Capítulos

  • 00:05Título
  • 02:02Preparation of the Bottom Layer: 0.6% Agarose Gel
  • 04:39Preparation of the Cell-containing Layer: 0.3% Agarose Gel
  • 06:44Preparation of the Feeder Layer: 0.3% Agarose Gel
  • 08:08Data Collection
  • 09:10Results: Colony Formation in BB-CLA-treated MCF10DCIS Cells
  • 10:40Conclusion

Summary

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Here, we document the use of the soft agar colony formation assay to test the effects of a peptidylarginine deiminase (PADI) enzyme inhibitor, BB-Cl-amidine, on breast cancer tumorigenicity in vitro.

Tags

Keywords: Soft Agar Colony Formation AssayTumor Progression3D Cellular EnvironmentCell ProliferationCell MigrationCell TransformationGenetic PerturbationsPADI2Breast CancerBB-Cl-amidineTumorigenicityMCF10DCIS Cells

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