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JoVE Journal Developmental Biology
Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord
 

Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord

Article DOI: 10.3791/52732-v • 08:26 min • May 14th, 2015
May 14th, 2015 •
1, 1,2
1Division of Genetics and Development, Toronto Western Research Institute and Krembil Neuroscience Center, 2Department of Surgery, Division of Neurosurgery, Toronto Western Hospital and University of Toronto

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Resumo
Automatic Translation

The adult mammalian spinal cord contains neural stem/progenitor cells (NSPCs) that can be isolated and expanded in culture. This protocol describes the harvesting, isolation, culture, and passaging of NSPCs generated from the periventricular region of the adult spinal cord from the rat and from human organ transplant donors.

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Keywords: Neural Stem Cells Neural Progenitor Cells Spinal Cord Adult Rat Human Neurosphere Proliferation Differentiation Neuron Astrocyte Oligodendrocyte Epidermal Growth Factor Fibroblast Growth Factor Heparin Neural Stem Cell Culture Neural Stem Cell Transplantation
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