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Muis Naïve CD4<sup> +</sup> T Cell Isolatie en<em> In vitro</em> Differentiatie in T cel subsets
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
DOI:

07:12 min

April 16, 2015

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Capítulos

  • 00:05Título
  • 01:09Mouse Lymph Node and Spleen Isolation
  • 02:11Tissue Processing and CD4+ Enrichment
  • 04:22Naïve CD4+ T Cell Sorting and In vitro Differentiation
  • 05:49Results: Representative In vitro Th Differentiation Data
  • 06:40Conclusion

Summary

Tadução automática

Naïve CD4+ T cells polarize to various subsets depending on the environment at the time of activation. The differentiation of naïve CD4+ T cells to various effector subsets can be achieved in vitro through the addition of T cell receptor stimuli and specific cytokine signals.

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