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癌症细胞球体机器人生产与药物测试的双水相系统

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

Robotic Production of Cancer Cell Spheroids with an Aqueous Two-phase System for Drug Testing

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癌症细胞球体机器人生产与药物测试的双水相系统

DOI:

10.3791/52754-v

•

09:58 min

•

April 23, 2015

•

Stephanie Lemmo Ham, Ehsan Atefi, Darcy Fyffe, Hossein Tavana

¹Department of Biomedical Engineering,The University of Akron

Capítulos

00:05Título
02:04Preparation of a Polymeric Aqueous Two Phase System
03:31Printing of Tumor Spheroids into a 96-well Plate
06:18Drug Treatment and Analysis of Viability in Cancer Cell Spheroids
08:01Results: Narrow Spheroid Size Distribution Is Essential for Drug Testing
09:04Conclusion

Summary

Tadução automática

A protocol for robotic printing of cancer cell spheroids in a high throughput 96-well plate format using an aqueous two-phase system is presented.

Tags

Keywords: Cancer Cell Spheroids In Vitro Tumor Model Aqueous Two-phase System High-throughput Drug Testing Robotic Liquid Handling Cell Viability Compound Screening

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