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Capítulos
- 00:05Título
- 02:12Protein Expression Using In Vitro Human Cell Free Expression System
- 03:21Purification of Expressed Recombinant Proteins
- 05:05Western Blotting
- 06:52Results: Purple Color Band on Nitrocellulose Paper During Developing Step Confirms the Successful Expression of Proteins
- 08:11Conclusion
Expression of malarial proteins in cell based systems remains challenging. We demonstrate two step and one step IVT (in vitro translation) cell free expression systems for expressing malarial recombinant rhoptry proteins from HeLa cells. We use a Ni-resin affinity based purification system to purify the rhoptry proteins.
