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HeLa Baseret Cell ytringsfrihed Systemer til ekspression af<em> Plasmodium</em> Rhoptry Proteiner
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Biologia
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JoVE Journal Biologia
HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins

HeLa Baseret Cell ytringsfrihed Systemer til ekspression af<em> Plasmodium</em> Rhoptry Proteiner

DOI:
10.3791/52772-v

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09:03 min

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June 10, 2015

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,
1Department of Biological, Geological, and Environmental Sciences,Cleveland State University

Capítulos

  • 00:05Título
  • 02:12Protein Expression Using In Vitro Human Cell Free Expression System
  • 03:21Purification of Expressed Recombinant Proteins
  • 05:05Western Blotting
  • 06:52Results: Purple Color Band on Nitrocellulose Paper During Developing Step Confirms the Successful Expression of Proteins
  • 08:11Conclusion

Summary

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Tadução automática

Ekspression af malaria proteiner i cellebaserede systemer stadig en udfordring. Vi demonstrerer to trin og et skridt IVT (in vitro translation) celle gratis ekspressionssystemer til at udtrykke malaria rekombinante rhoptry proteiner fra HeLa-celler. Vi bruger en Ni-harpiks affinitet baseret rensningssystemet for at oprense rhoptry proteiner.

Tags

HeLa Cell-free ExpressionPlasmodium ProteinsMalariaVaccine CandidatesCell-free Expression SystemsProtein ExpressionIn Vitro Human Cell-free ExpressionPF3D7 0114100PF3D7 0925900PF3D7 1361800Protein YieldAffinity Purification

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