/

Imunologia e Infecção

/

方法来提高高分辨率的敏感性疟疾熔单核苷酸多态性基因分型

JoVE Journal
Imunologia e Infecção

This content is Free Access.

JoVE Journal Imunologia e Infecção

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

Please note that all translations are automatically generated. Click here for the English version.

方法来提高高分辨率的敏感性疟疾熔单核苷酸多态性基因分型

DOI:

10.3791/52839-v

•

10:27 min

•

November 10, 2015

•

Rachel Daniels², Elizabeth J. Hamilton, Katelyn Durfee, Daouda Ndiaye, Dyann F. Wirth⁵, Daniel L. Hartl, Sarah K. Volkman⁴

¹Department of Organismic and Evolutionary Biology,Harvard University, ²Department of Immunology and Infectious Diseases,Harvard T.H. Chan School of Public Health, ³Faculty of Medicine and Pharmacy,Cheikh Anta Diop University, ⁴School of Nursing and Health Sciences,Simmons College, ⁵Institute of Infectious Diseases,Broad Institute

Capítulos

00:05Título
01:58Template Preparation
03:46PCR Amplification
05:37HRM Analysis
08:26Results: Blocked-probe-based and MAAB Increases HRM Genotyping Sensitivity
09:46Conclusion

Summary

Tadução automática

虽然高分辨率熔解分析提供了一个异质群体的单核苷酸多态性区分的能力，突变等位基因扩增偏置可提高其检测存在于样品中相对较低的百分比等位基因的能力。本协议描述的改进，提高了高分辨率熔解分析的灵敏度。

Tags

Keywords: Malaria Plasmodium Single-nucleotide Polymorphisms (SNPs)Drug Resistance Population Surveillance High-resolution Melting (HRM) Analysis Polygenomic Infections PCR Amplification Minor Allele Genotyping Sensitivity

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

生产的灭鼠疟疾寄生虫的遗传交叉议定书“

两种异核体的形成方法，发现丙型肝炎病毒的制约因素

疟疾蚊子中的二维和三维染色体绘画

建立和高通量安装程序研究优化<em>表皮葡萄球菌</em>和<em>分枝杆菌</em>感染作为模型药物发现

使用复分析定性测量细菌生产和敏感性，以肺炎球菌细菌素

的高分辨电子显微<em>幽门螺杆菌</em>恰IV型分泌系统的霹雳产于铁可用性的条件变

一个<em>体外</em>模型在HIV合并感染的背景下测量免疫应答疟疾

方法来抑制细菌Pyomelanin生产和确定敏感性氧化应激的影响相应增加

中性粒细胞分离和分析，以确定他们的淋巴瘤细胞的敏感性对治疗药物的作用

对于血液处理快速的方法，以提高血浆肽水平的人血产量

Read Article