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室管膜纤毛在小鼠脑侧脑室实时成像

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Live Imaging of the Ependymal Cilia in the Lateral Ventricles of the Mouse Brain

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室管膜纤毛在小鼠脑侧脑室实时成像

DOI:

10.3791/52853-v

•

06:58 min

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June 01, 2015

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Alzahra J. Al Omran, Hannah C. Saternos, Tongyu Liu, Surya M. Nauli, Wissam A. AbouAlaiwi

¹Department of Pharmacology and Experimental Therapeutics,University of Toledo, College of Pharmacy and Pharmaceutical Sciences, ²Life Sciences Institute,University of Michigan, ³Department of Biomedical & Pharmaceutical Sciences,Chapman University, School of Pharmacy, Rinker Health Science campus

Capítulos

00:05Título
01:31Brain Extraction, Sectioning, and Tissue Preparation
02:28Live Imaging Configuration and Setup
04:00Calcium Signal Recording and Immunofluorescence Microscopy
05:26Results: Measuring Ependymal Cilia Function and Calcium Signaling
06:22Conclusion

Summary

Tadução automática

使用高分辨率微分干涉对比（DIC）显微镜，离体观察位于小鼠脑室内游动室管膜纤毛的跳动的证明通过实时成像。该技术允许独特纤毛跳动频率的记录和跳动角度以及它们的细胞内钙振荡起搏属性。

Tags

Keywords: Live Imaging Ependymal Cilia Lateral Ventricles Mouse Brain Multiciliated Ependymal Cells Ciliary Dynamics Ciliary Beating Frequency Calcium Oscillations Immunofluorescence Imaging Neurological Deficits

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