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SpOT the Correct Tissue Every Time in Multi-tissue Blocks
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Biologia
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JoVE Journal Biologia
SpOT the Correct Tissue Every Time in Multi-tissue Blocks

SpOT the Correct Tissue Every Time in Multi-tissue Blocks

DOI:
10.3791/52868-v

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06:53 min

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May 31, 2015

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, ,
1Center for Advanced Preclinical Research (CAPR),Frederick National Laboratory for Cancer Research, 2Leidos Biomedical Research, Inc., 3Department of Oncology,Georgetown University

Capítulos

  • 00:05Título
  • 01:22Construction of Specimen Orientation Tags (SpOTs)
  • 03:55Using SpOTs
  • 05:41Tissue Sections with SpOTs Technology
  • 06:16Conclusion

Summary

Tadução automática

Tadução automática

The purpose of the Specimen Orientation Tag (SpOT) is to function as an orientation tool to aid in individual tissue identification in multi-tissue paraffin blocks. These protocols demonstrate how it is constructed easily from common, low-cost histology materials and serves as a reliable visual marker in paraffin blocks and sections.

Tags

Non-arrayed BlocksSpecimen Orientation Tag (SpOT)Histology LaboratoryHigh Throughput AnalysisExperimental UniformityReduced Time And Cost

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