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Capítulos
- 00:05Título
- 02:24Irradiation and Splenocyte Harvest
- 04:05Splenocyte Culture Preparation
- 05:43Irradiation Challenge
- 06:39Immunofluorescent Labeling with Anti-gammaH2AX Antibody
- 08:12Results: Flow Cytometric gammaH2AX Detection is Sensitive and Specific
- 10:08Conclusion
A protocol to evaluate changes in DNA damage levels and DNA repair capacity that may be induced by chronic in vivo low dose irradiation in mouse spleen lymphocytes, by measuring phosphorylated histone H2AX, a marker of DNA double-strand breaks, using flow cytometry is presented.
