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Корреляционный конфокальной и 3D Электронная микроскопия конкретного чувственного Cell
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell
DOI:

08:00 min

July 19, 2015

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Capítulos

  • 00:05Título
  • 01:34Harvesting, Dissecting, and Fixing Tissue
  • 02:50Infiltrating and Embedding Tissue Sections in Resin
  • 04:17Optimizing SBEM Images for Surface Segmentation
  • 05:33Results: Correlative Confocal and SEM Analysis of Epithelial Cell Ultrastructure
  • 07:19Conclusion

Summary

Tadução automática

Here, we introduce a method, cocem3D, to unveil the ultrastructure of a specific cell in its native tissue by bridging confocal and serial block-face scanning electron microscopy.

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