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Capítulos
- 00:05Título
- 01:03Preparing Reaction Mix for Bulk Droplet Readout
- 02:47Droplet Formation
- 04:05Isothermal Amplification, Data Acquisition and Analysis
- 05:12Results: Quantification of Nucleic Acids with a Simplified Readout
- 06:33Conclusion
We describe an endpoint digital assay for quantifying nucleic acids with a simplified (analog) readout. We measure bulk fluorescence of droplet-based digital assays using a standard qPCR machine rather than specialized instrumentation and confirm our results by microscopy.
