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Rodent Brain Microinjection to Study Molecular Substrates of Motivated Behavior
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JoVE Journal Comportamento
Rodent Brain Microinjection to Study Molecular Substrates of Motivated Behavior

Rodent Brain Microinjection to Study Molecular Substrates of Motivated Behavior

DOI:
10.3791/53018-v

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10:05 min

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September 16, 2015

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, , ,
1Virginia Institute for Psychiatric and Behavioral Genetics, Department of Psychiatry,Virginia Commonwealth University School of Medicine, 2Institute for Drug and Alcohol Studies, Departments of Psychiatry, Pharmacology, and Neuroscience,Virginia Commonwealth University School of Medicine

Capítulos

  • 00:05Título
  • 01:13Preparation of Cannula and Obturator
  • 03:13Preparation of Microinjector
  • 05:32Performing Microinjection
  • 08:50Results: Reduced Rat Motivation to Self-administer Ethanol by Overexpressing DREADDs in Nucleus Accumbens Core Astrocytes
  • 09:35Conclusion

Summary

Tadução automática

Tadução automática

Rodents are an appropriate model to investigate the molecular substrates of behavior and complex psychiatric disorders. Brain microinjection in awake rodents can be used to elucidate disease substrates. An efficient and customizable brain microinjection method as well as the execution of an operant paradigm that quantifies motivation is presented.

Tags

Keywords: Brain MicroinjectionMolecular SubstratesMotivated BehaviorRodent ModelProgressive RatioOperant ConditioningReinforcementBehavioral Paradigm

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