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在II-45同基因大鼠模型间皮瘤原位移植和外周免疫细胞监视

JoVE Journal
Medicina

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JoVE Journal Medicina

Orthotopic Implantation and Peripheral Immune Cell Monitoring in the II-45 Syngeneic Rat Mesothelioma Model

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在II-45同基因大鼠模型间皮瘤原位移植和外周免疫细胞监视

DOI:

10.3791/53019-v

•

09:31 min

•

October 02, 2015

•

Chris J. Weir, Amanda L. Hudson, Lyndsay Peters, Viive M. Howell

¹Bill Walsh Translational Cancer Research Laboratory, Kolling Institute of Medical Research,Royal North Shore Hospital, University of Sydney, ²Northern Blood Research Laboratory, Kolling Institute of Medical Research,Royal North Shore Hospital, University of Sydney

Capítulos

00:05Título
01:44In Vivo Cell Implantation
03:46Tail Vein Blood Collection
05:04Bead-based Immune Cell Profiling and Analysis
06:42Results: Representative Circulating Immune Cell Counts from Rats with Mesothelioma
08:35Conclusion

Summary

Tadução automática

胸膜恶性间皮瘤由II-45间皮瘤细胞进入胸膜免疫活性大鼠腔植入的原位大鼠模型的产生提出。甲流式细胞法分析从25微升血样在这些动物7免疫细胞亚群也被描述。

Tags

Orthotopic Implantation Peripheral Immune Cell Monitoring Syngeneic Rat Mesothelioma Model Immune-based Treatments Tumor Microenvironment Flow Cytometry Lymphocytes Monocytes Neutrophils T-cells B-cells Natural Killer Cells Neutrophil To Lymphocyte Ratio

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