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实施膜片钳和活荧光显微镜监视新鲜分离的PKD上皮细胞的功能特性
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Biologia
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JoVE Journal Biologia
Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium
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实施膜片钳和活荧光显微镜监视新鲜分离的PKD上皮细胞的功能特性

DOI:
10.3791/53035-v

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08:46 min

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September 01, 2015

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, , , , ,
1Department of Physiology,Medical College of Wisconsin, 2Department of Integrative Biology & Pharmacology,University of Texas Health Science Center at Houston

Capítulos

  • 00:05Título
  • 02:14Isolation of Renal Cysts and Connecting Tubules/Collecting Duct Segments
  • 05:02Single Channel Patch-clamp Electrophysiology
  • 05:32Ratiometric Epifluorescence Measurement of Intracellular Calcium Concentration in the Epithelial Cells
  • 06:55Results: Effect of Benzamil Treatment and ATP in the Cystic Cells of PCK Rats
  • 08:08Conclusion

Summary

Tadução automática

Tadução automática

表现在肾小管上皮细胞离子通道在多囊肾病的病理一个显著的作用。这里，我们描述用于执行囊性上皮从啮齿动物肾脏新鲜分离的膜片钳分析和细胞内钙水平的测量的实验方案。

Tags

Patch ClampLive Fluorescence MicroscopyPolycystic Kidney DiseasePKDARPKDIon ChannelsCalcium SignalingEpithelial MonolayerEx Vivo AnalysisPCK RatSprague Dawley RatNative SettingEpithelial IsolationImmunostainingPrimary CultureBiochemical Assays

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