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Photopatterning Proteins and Cells in Aqueous Environment Using TiO2 Photocatalysis
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1Frontier Research Institute for Interdisciplinary Sciences,Tohoku University, 2CREST,Japan Science and Technology Agency, 3School of Fundamental Science and Engineering,Waseda University, 4Research Institute of Electrical Communication,Tohoku University, 5Graduate School of Biomedical Engineering,Tohoku University, 6Faculty of Science and Engineering,Waseda University
Capítulos
- 00:05Título
- 01:46Preparation of Titanium Dioxide-coated Glass Coverslip
- 03:16Surface Coating with Cell-repellent Film
- 04:43Ex Situ Surface Patterning
- 06:46Cell Culture and In Situ Surface Patterning
- 08:36Results: Surface Modification Enhances Protein-based Cell Adhesion
- 09:59Conclusion
We describe a protocol for modifying cell affinity of a scaffold surface in aqueous environment. The method takes advantage of titanium dioxide photocatalysis to decompose organic film in the photo-irradiated region. We show that it can be used to create microdomains of scaffolding proteins, both ex situ and in situ.
