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العدوى فيفو السابقين الفئران البشرة مع فيروس الهربس البسيط من النوع 1
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1
DOI:

11:56 min

August 24, 2015

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Capítulos

  • 00:05Título
  • 01:16Preparation of Instruments and Culture Medium
  • 02:28Preparation of Epidermal Sheets from Murine Newborn Skin or from Adult Tail Skin
  • 04:31Dissociation of Epidermis for FACS Analysis
  • 05:57Infection of Epidermal Sheets with Herpes Simplex Virus Type 1 and Visualization of Infected Cells
  • 09:36Results: Herpes Simplex Virus Infection of Murine Epidermal Sheets
  • 11:20Conclusion

Summary

Tadução automática

The skin is one target tissue of the human pathogen herpes simplex virus type 1 (HSV-1). To explore the invasion route of HSV-1 into tissue, we established an ex vivo infection model of murine epidermal sheets which represent the outermost layer of skin.

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