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El estudio de la función de proteínas y el Papel de la expresión de la proteína alterada por la interferencia de anticuerpos y reconstrucciones tridimensionales
JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia
Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions
DOI:

11:57 min

April 21, 2016

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Capítulos

  • 00:05Título
  • 01:23Preparations
  • 02:36Chariot Complex Formation
  • 03:51Transfecting the Cells
  • 05:41Imaging and Reconstruction
  • 09:35Results: Antibody Interference in HEK293 Cells and Primary Hippocampal Neurons
  • 11:08Conclusion

Summary

Tadução automática

Controlling protein expression is not only essential to every organism alive, but also an important strategy to investigate protein functions in cellular models. The protocol presented shows the application of antibody interference in mammalian cells including primary hippocampal neurons and demonstrates the use of three-dimensional reconstructions in studying protein function.

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