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Een 3D Human longweefsel Model voor functionele studies op<em> Mycobacterium tuberculosis</em> Infectie
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
A 3D Human Lung Tissue Model for Functional Studies on Mycobacterium tuberculosis Infection
DOI:

10:10 min

October 05, 2015

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Capítulos

  • 00:05Título
  • 01:13Fibroblast-collagen Matrix Continuous Culture
  • 03:35Immune and Lung Epithelial Cell Seeding
  • 05:423D Lung Tissue Model Air Exposure, Harvest and Mounting
  • 07:123D Model Visualization, Acquisition and Quantitative Analysis
  • 08:26Results: Representative 3D Visualization and Quantitative Tissue Model Analysis
  • 09:39Conclusion

Summary

Tadução automática

Human tuberculosis infection is a complex process, which is difficult to model in vitro. Here we describe a novel 3D human lung tissue model that recapitulates the dynamics that occur during infection, including the migration of immune cells and early granuloma formation in a physiological environment.

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