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3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation
JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia
3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation

3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation

DOI:
10.3791/53085-v

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12:37 min

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October 07, 2015

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, , ,
1Orthopaedic Surgery Department,Stanford University, 2Mechanical Engineering Department,Stanford University, 3Bioengineering Department,Stanford University

Capítulos

  • 00:05Título
  • 02:15Articular Chondrocyte Isolation
  • 03:54Biomimetic Hydrogel Fabrication
  • 05:43Cell Encapsulation
  • 08:59Mechanical Testing
  • 09:54Results: Bioactive Hydrogels Support Growth and Maturation of Human Articular Chondrocytes
  • 11:23Conclusion

Summary

Tadução automática

Tadução automática

Cartilage repair represents an unmet medical challenge and cell-based approaches to engineer human articular cartilage are a promising solution. Here, we describe three-dimensional (3D) biomimetic hydrogels as an ideal tool for the expansion and maturation of human articular chondrocytes.

Tags

Keywords: 3D HydrogelArticular ChondrocyteCartilage RegenerationCell CultureJuvenileAdultOsteoarthriticChondroitin SulfateGene ExpressionBiochemicalBiomechanical

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