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Sandwich-like Microenvironments to Harness Cell/Material Interactions
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JoVE Journal Bioengenharia
Sandwich-like Microenvironments to Harness Cell/Material Interactions

Sandwich-like Microenvironments to Harness Cell/Material Interactions

DOI:
10.3791/53090-v

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06:50 min

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August 04, 2015

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, ,
1Center for Biomaterials and Tissue Engineering,Universitat Politècnica de València, 2Division of Biomedical Engineering, School of Engineering,University of Glasgow, 3Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN)

Capítulos

  • 00:05Título
  • 01:27Production of Dorsal Substrates
  • 02:52Sandwich Culture
  • 03:55Wound Healing Assay
  • 04:55Results: Sandwich-like Culture Strongly Influences Cell Morphology, Cell Differentiation and ECM Reorganization
  • 06:18Conclusion

Summary

Tadução automática

Tadução automática

The following protocol describes the procedure to assemble sandwich-like cultures to be used as an intermediate stage between bi-dimensional (2D) and three-dimensional (3D) cellular environments. The engineered systems can have applications in microscopy, biomechanics, biochemistry and cell biology assays.

Tags

Cell-material Interactions3D Cell Culture2D Cell CultureSandwich-like MicroenvironmentsCell Surface ReceptorsExtracellular MatrixOutside-in SignalingCell BehaviorVersatile ToolTopographyStiffnessProtein Coatings

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