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A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
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Bioengenharia
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JoVE Journal Bioengenharia
A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

DOI:
10.3791/53120-v

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09:09 min

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November 23, 2015

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, , , ,
1Materials Science and Technology,Naval Research Laboratory, 2Center for Bio/Molecular Science and Engineering,Naval Research Laboratory

Capítulos

  • 00:05Título
  • 01:05Nanostructure Fabrication
  • 03:23Chip Cleaning and Application of Self-assembled Monolayer
  • 04:07LSPR General Settings – Chip Preparation and Mounting
  • 06:39LSPR Imaging of Anti-c-myc Secretions from 9E10 Hybridoma Cells
  • 07:14Results: Spatio-temporal Imaging of Single Cell Secretions by LSPRi
  • 08:43Conclusion

Summary

Tadução automática

Tadução automática

Inter-cellular communication is critical for controlling various physiological activities within and outside the cell. This paper describes a protocol for measuring the spatio-temporal nature of single cell secretions. To achieve this, a multidisciplinary approach is used which integrates label-free nanoplasmonic sensing with live cell imaging.

Tags

Keywords: Label-freeSingle Cell SecretionsLocalized Surface Plasmon Resonance Imaging (LSPRi)Protein SecretionCell-to-cell SignalingNanofabricationBiofunctionalizationBinding KineticsLive Cell Imaging

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