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Фотоактивированные Локализация микроскопия с Бимолекулярные флуоресценции комплементации (BiFC-PALM)
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Capítulos
- 00:05Título
- 00:40Imaging Fixed Cells
- 03:57Image Processing
- 07:46Track Single Molecules in Live Cells
- 08:55Results: Visualization of Individual Ras-Raf Interactions Using BiFC-PALM
- 11:02Conclusion
Protein-protein interactions are visualized in cells with nanometer spatial resolution by combining bimolecular fluorescence complementation (BiFC) with photoactivated localization microscopy (PALM). Described here is the use of BiFC-PALM for imaging Ras-Raf interactions in U2OS cells for visualizing the nanoscale clustering and diffusion of individual Ras-Raf complexes.
