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鸡胚全视网膜外植体电穿孔和化学试剂处理
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments
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鸡胚全视网膜外植体电穿孔和化学试剂处理

DOI:
10.3791/53202-v

•

09:40 min

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September 30, 2015

•

, ,
1Department of Neuroscience, Biomedical Center,Uppsala University

Capítulos

  • 00:05Título
  • 01:33Egg Handling and Eye Collection
  • 03:21Preparation of Whole Retinal Explants
  • 04:15Electroporation of Whole Retinal Explants
  • 07:28Results: Uses of Cultured Whole Retinal Explants
  • 08:59Conclusion

Summary

Tadução automática

Tadução automática

本协议描述的方法来剖析，实验操作和鸡胚培养全视网膜植。当高的成功率，有效性和再现性都需要测试质粒电穿孔和/或试剂的物质， 即，酶抑制剂的效应的外植体培养是有用的。

Tags

Whole Retinal ExplantsChicken EmbryosElectroporationChemical ReagentsExperimental ModelCentral Nervous SystemIntraocular InjectionsEx Vivo CultureControlled ExposureRapidReproducibleCost-effective

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