A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

00:05Título
01:22Cell Isolation from Human Placenta
03:47Harvesting Human Placenta-derived Cell Conditioned Medium
05:18Culture of Human Embryonic Stem Cells and Induction of Pluripotent Stem Cells
05:59Results: hPSC Culture in Human Placental-derived Cell Culture Medium Mimics that of the Gold Standard
07:05Conclusion

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This protocol provides a simple and efficient way to propagate human pluripotent stem cells (hPSCs) using only conditioned media derived from the human placenta in a gelatin-coated dish without additional exogenous supplementation or hPSC-specific synthetic substrata.

一种新的文化模式的人多能干明胶胎盘条件培养基细胞增殖

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