Isolamento de mitocôndrias de quantidades mínimas de Rato Músculo Esquelético para medições High Throughput Microplate respiratórias

Here, we present a modification of a previously reported method that allows for the isolation of high quality and purified mitochondria from smaller quantities of mouse skeletal muscle. This procedure results in highly coupled mitochondria that respire with high function during microplate based respirometirc assays.