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Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease
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JoVE Journal Medicina
Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease

Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease

DOI:
10.3791/53250-v

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06:04 min

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February 09, 2016

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, , , , ,
1School of Biomedical Science and Pharmacy,University of Newcastle

Capítulos

  • 00:05Título
  • 00:40Gastrointestinal Tract Harvest
  • 02:03Permeability Measurement
  • 04:13Results: Representative Intestinal Permeability Investigations
  • 05:09Conclusion

Summary

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This protocol describes the use of excised intestinal tissue preparations or "intestinal sacs" as an ex vivo model of intestinal barrier function. This model may be used to assess integrity of both the epithelial barrier and the mucous gel layer at specific intestinal sites in animal models of digestive disease.

Tags

Ex VivoIntestinal SacsMucosal PermeabilityGastrointestinal DiseaseIntestinal IntegrityEpithelial BarrierIntestinal AbsorptionIn Vitro Cell CultureIn Vivo AssessmentSite-specific PermeabilityMucous Gel LayerTight Junctional ProteinsPeyer’s PatchesIntestinal TractDuodenumJejunumColonIleum

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