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Konstruktion af Cell-baserede Neurotransmitter Fluorescerende Engineered Journalister (CNiFERs) for Optical Påvisning af Neurotransmittere<em> In vivo</em
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JoVE Journal Neurociência
Construction of Cell-based Neurotransmitter Fluorescent Engineered Reporters (CNiFERs) for Optical Detection of Neurotransmitters In Vivo

Konstruktion af Cell-baserede Neurotransmitter Fluorescerende Engineered Journalister (CNiFERs) for Optical Påvisning af Neurotransmittere<em> In vivo</em

DOI:
10.3791/53290-v

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12:48 min

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May 12, 2016

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, , , ,
1Department of Neuroscience,Icahn School of Medicine at Mount Sinai, 2Department of Physics,University of California, San Diego, 3Section of Neurobiology,University of California, San Diego

Capítulos

  • 00:05Título
  • 00:56Lentiviral Transduction of HEK293/TN-XXL/Gqi5 Cells
  • 02:49Preparation for FACS and Isolation of the Single CNiFER Clones
  • 05:17CNiFER Implantation into Mouse Cortex
  • 07:52In Vivo Imaging of CNiFER Clones
  • 10:42Results: Examples of Flexstation Analysis, Agonist-induced FRET Response and Dose Response Curves for D2 CNiFER
  • 12:02Conclusion

Summary

Tadução automática

Tadução automática

Vi præsenterer en protokol til at skabe celle-baserede neurotransmitter fluorescerende manipuleret reportere (CNiFERs) til optisk detektion af volumetrisk neurotransmitter frigørelse.

Tags

Cell-based Neurotransmitter Fluorescent Engineered Reporters (CNiFERs)Optical DetectionNeurotransmittersIn VivoHEK293 CellsLentivirusFACS AnalysisAgonist CurveGPCRTNXXLFRETFluorometric Plate Reader

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