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Isolatie van virale replicatie compartiment verrijkt Sub-nucleaire fracties uit adenovirus-geïnfecteerde normale menselijke cellen
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Capítulos
- 00:05Título
- 00:57Preparation of the Subnuclear Fraction Enriched in Adenoviral Replication Compartments
- 05:54Immunofluorescence Visualization of RCf
- 08:00Results: Presence of Adenoviral Components in the Replication Compartment Fraction from Infected Human Foreskin Fibroblasts
- 08:49Conclusion
We provide a novel strategy to isolate viral replication compartments (RC) from adenovirus (Ad)-infected human cells. This approach represents a cell-free system that can help to elucidate the molecular mechanisms regulating viral genome replication and expression as well as regulation of viral-host interactions established at the RC.
