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Una alternativa semplice per iniezione stereotassica per Brain Knockdown specifico miRNA
JoVE Journal
Neurociência
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JoVE Journal Neurociência
A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA
DOI:

06:53 min

December 26, 2015

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Capítulos

  • 00:00Título
  • 01:52Antagomir Design Strategy
  • 02:10Antagomir-peptide Complex Preparation
  • 03:08Tail Vein Injection of Antagomir-peptide Complex
  • 03:29Behavioural Assays: Hind-limb Clasping
  • 03:48Behavioural Assays: Ledge Test
  • 04:03Behavioural Assays: Footprint Assay
  • 04:32Representative Results
  • 06:21Conclusion

Summary

Tadução automática

MicroRNAs play crucial roles in the brain and are potential targets for modeling neuro-degeneration. However, perturbing miRNA levels is challenging due to the short length of miRNA and inaccessibility of the brain tissue. This video presents a method for antagomir design and brain specific delivery using a neuropeptide in mice.

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